Journal: Current Issues in Molecular Biology

Article Title: IGFBP5 Promotes Atherosclerosis in APOE −/− Mice Through Phenotypic Transformation of VSMCs

doi: 10.3390/cimb47070555

Figure Lengend Snippet: Overexpression of IGFBP5 does not influence food consumption by and the body weight of ApoE −/− mice. ( A ) IGFBP5 mRNA expression level in skeletal muscle of ApoE −/− mice at 10 weeks of experimental treatment ( n = 3). ( B ) Plasma IGFBP5 protein levels at 10 weeks of experimental treatment ( n = 3). ( C ) Weight change curve ( n = 15). ( D ) Comparison of average daily food intake during the entire experimental treatment among groups ( n = 15). Data are expressed as the mean ± SEM. * p < 0.05. NS means no significant difference.

Article Snippet: The primary antibody used in this experiment was a rabbit anti- IGFBP5 antibody (1:500, #55205-1-AP; Proteintech Group, Chicago, IL, USA).

Techniques: Over Expression, Expressing, Clinical Proteomics, Comparison

Journal: Current Issues in Molecular Biology

Article Title: IGFBP5 Promotes Atherosclerosis in APOE −/− Mice Through Phenotypic Transformation of VSMCs

doi: 10.3390/cimb47070555

Figure Lengend Snippet: Overexpression of IGFBP5 enhances the development of atherosclerotic plaques in ApoE −/− mice. Oil Red O staining of the aorta ( A ) and statistical quantification ( B ). ( C ) H&E staining (up panel) and Oil Red O staining (down panel) of cardiac outflow tract sections. Scale bar: 500 μm. ( D ) Statistical quantification of neutral lipids in the plaques of the cardiac outflow tract sections stained with Oil Red O ( n = 10). Data are expressed as the mean ± SEM. * p < 0.05. NS means no significant difference.

Article Snippet: The primary antibody used in this experiment was a rabbit anti- IGFBP5 antibody (1:500, #55205-1-AP; Proteintech Group, Chicago, IL, USA).

Techniques: Over Expression, Staining

Journal: Current Issues in Molecular Biology

Article Title: IGFBP5 Promotes Atherosclerosis in APOE −/− Mice Through Phenotypic Transformation of VSMCs

doi: 10.3390/cimb47070555

Figure Lengend Snippet: Effect of overexpression of IGFBP5 on blood lipid levels in ApoE −/− mice. Plasma was collected from each group of mice ( n = 15) at week 0, 4, 8, and 12 of treatment to measure total cholesterol (TC) ( A ), total triglycerides (TG) ( B ), low-density lipoprotein cholesterol (LDL-C) ( C ), and high-density lipoprotein cholesterol (HDL-C) ( D ) levels. Data are expressed as the mean ± SEM.

Article Snippet: The primary antibody used in this experiment was a rabbit anti- IGFBP5 antibody (1:500, #55205-1-AP; Proteintech Group, Chicago, IL, USA).

Techniques: Over Expression, Clinical Proteomics

Journal: Current Issues in Molecular Biology

Article Title: IGFBP5 Promotes Atherosclerosis in APOE −/− Mice Through Phenotypic Transformation of VSMCs

doi: 10.3390/cimb47070555

Figure Lengend Snippet: Transcriptome analysis of aortic tissues in ApoE −/− mice following overexpression of IGFBP5 . ( A , B ) Heatmap representing global gene expression in aortic tissues of AAV-GFP and AAV- IGFBP5 mice after 12 weeks of being fed a HFD ( n = 4).

Article Snippet: The primary antibody used in this experiment was a rabbit anti- IGFBP5 antibody (1:500, #55205-1-AP; Proteintech Group, Chicago, IL, USA).

Techniques: Over Expression, Gene Expression

Journal: Current Issues in Molecular Biology

Article Title: IGFBP5 Promotes Atherosclerosis in APOE −/− Mice Through Phenotypic Transformation of VSMCs

doi: 10.3390/cimb47070555

Figure Lengend Snippet: Gene Ontology analyses of the differentially expression genes in aortic tissues in ApoE −/− mice following overexpression of IGFBP5 . ( A ) Gene Ontology analyses of the differentially expression genes in aortic of AAV-GFP and AAV- IGFBP5 mice after 12 weeks of HFD ( n = 4). ( B ) GO analysis was performed based on three categories: biological process (BP), molecular function (MF), and cellular component (CC).

Article Snippet: The primary antibody used in this experiment was a rabbit anti- IGFBP5 antibody (1:500, #55205-1-AP; Proteintech Group, Chicago, IL, USA).

Techniques: Expressing, Over Expression

Journal: Current Issues in Molecular Biology

Article Title: IGFBP5 Promotes Atherosclerosis in APOE −/− Mice Through Phenotypic Transformation of VSMCs

doi: 10.3390/cimb47070555

Figure Lengend Snippet: Overexpression of IGFBP5 induces VSMCs to adopt a proliferative state. ( A ) Flow cytometric analysis of cell cycle distribution of immortalized mouse vascular smooth muscle cell line treated with different concentrations of IGFBP5 during proliferation ( n = 3). ( B ) Cell cycle distribution statistics ( n = 3). ( C ) The CCK-8 kit was used to detect the effect of different concentrations of IGFBP5 on VSMC proliferation for 24 h ( n = 5). ( D ) Effects of different concentrations of IGFBP5 on cell migration in cell scratch assays ( n = 3). ( E ) Statistics of relative distance of cell migration ( n = 3). ( F ) Real-time qPCR analysis of ACTA2 , MYH11 , TAGLN , CNN1 and KLF4 transcription expression in immortalized mouse vascular smooth muscle cells treated with different concentrations of IGFBP5 ( n = 3). GAPDH was used as a control. Data are expressed as the mean ± SEM. * p < 0.05.

Article Snippet: The primary antibody used in this experiment was a rabbit anti- IGFBP5 antibody (1:500, #55205-1-AP; Proteintech Group, Chicago, IL, USA).

Techniques: Over Expression, CCK-8 Assay, Migration, Expressing, Control

Journal: Oncotarget

Article Title: New therapeutic approach to heart failure due to myocardial infarction based on targeting growth hormone-releasing hormone receptor

doi:

Figure Lengend Snippet: TaqMan ® probes used to examine the impact of treatment with GHRH analogs on mRNA levels of extracellular matrix (ECM) related genes

Article Snippet: IGFBP5 , Rn00563116_m1.

Techniques:

Journal: Scientific Reports

Article Title: Tracing recombinant bovine somatotropin ab(use) through transcriptomics: the potential of bovine somatic cells in a multi-dose longitudinal study

doi: 10.1038/s41598-019-41343-6

Figure Lengend Snippet: List of genes selected to evaluate their expression in somatic cells.

Article Snippet: IGFBP5 , Insulin like growth factor binding protein 5 , NM_001105327.2 , bt03258785-g1 , 62.

Techniques: Expressing, Sequencing, Amplification, Binding Assay